Single cell transcriptomics of Ewing Sarcoma cell lines. Single cell transcriptomics of Ewing Sarcoma cell lines

We report single cell gene expression profiles for Ewing Sarcoma cell lines CHLA9, CHLA10, and TC71. Cell lines were harvested and prepared as single cell suspensions with high viability. Library prep was performed with the 10X Genomics v3.1 3' kit. All samples were sequenced on the illumina HiSeq 3000 with a 28+8+100 configuration. CHLA9 and CHLA10 libraries were re-sequenced on the Illumina NovaSeq with a 28+8+91 configuration. Counts were processed with cellranger v3.1.0. Both the raw data and the cellranger output are provded. Overall design: 10X Genomics single cell RNA-Seq (scRNA-Seq) of Ewing Sarcoma cell lines CHLA9, CHLA10, and TC71

本研究报道了尤因肉瘤（Ewing Sarcoma）细胞系CHLA9、CHLA10及TC71的单细胞基因表达谱。所用细胞系经收获处理后，被制备为高存活率的单细胞悬液。文库构建采用10X Genomics v3.1 3'端试剂盒完成。所有样本均在因美纳（Illumina）HiSeq 3000测序平台上进行测序，测序配置为28+8+100。CHLA9与CHLA10的文库额外采用因美纳NovaSeq测序平台进行重测序，测序配置为28+8+91。计数数据通过Cell Ranger（cellranger）v3.1.0进行分析处理。本研究同时提供原始测序数据与Cell Ranger分析输出结果。实验整体设计：针对尤因肉瘤细胞系CHLA9、CHLA10及TC71开展10X Genomics单细胞RNA测序（single cell RNA-Seq，缩写scRNA-Seq）。