Bacterial strains, plasmids, and primers used in this study.

aKmR, ApR and StrR = kanamycin, ampicillin and streptomycin resistance, respectively.bUnderlined nucleotides are restriction sites added and the restriction enzymes are indicated at the end of primers.

aKmR、ApR及StrR分别对应卡那霉素（kanamycin）抗性、氨苄青霉素（ampicillin）抗性与链霉素（streptomycin）抗性；b 下划线标注的核苷酸为添加的限制性酶切位点（restriction site），引物（primer）末端标注了所使用的限制性内切酶（restriction enzyme）。