Dynamic regulation of miRNA accumulation during C. elegans larval development [smallRNA-Seq 4]

Here, we profile miRNA expression at high temporal resolution (1 hr interval) during C. elegans postembryonic development, which identifies several miRNAs with highly dynamic concentration changes Synchronized L1's of N2 and HW2643 (mir-235(xe181[seed_mutant]) were plated on 10 cm plates (2000 worms/plate) and kept at 25°C for the time-course. Every hour samples were collected from 18h to 30h, extracted RNA, treated with DNAse and subjected to small-RNA sequencing

本研究以1小时间隔的高时间分辨率，对秀丽隐杆线虫（C. elegans）胚胎后发育过程中的微小RNA（microRNA, miRNA）表达谱进行分析，鉴定出多个浓度变化呈现高度动态特征的miRNA。将同步化的N2品系与HW2643品系（mir-235(xe181[seed_mutant])）的L1期线虫接种于10 cm培养皿中，每皿接种2000条线虫，随后置于25℃条件下开展时间序列培养。在发育18小时至30小时期间，每小时采集一次样本，提取样本RNA并经脱氧核糖核酸酶（DNAse）处理后，进行小RNA测序（small-RNA sequencing）。