Table_2_Characterization and genomic Analysis of a novel Pseudomonas phage vB_PsaP_M1, representing a new viral family, Psaeviridae.xlsx

Pseudomonas is a ubiquitous and ambiguous opportunistic pathogen, and plays an important ecological role in the ocean. Here, a new species, Pseudomonas phage vB_PsaP_M1, is described, which was isolated from the surface coastal waters of Qingdao, China. vB_PsaP_M1 contains a linear, double-stranded 89,387-bp genome with a GC content of 41.04% and encoding 184 putative open reading frames (ORFs). There were 50 conservative domains were predicted with BLASTp, including two auxiliary metabolic genes (Phosphate-inducible gene phoH and signal peptide peptidase A, sppA). Phylogenetic analysis of whole genome amino acid sequence and comparative genomic analysis showed that vB_PsaP_M1 has a distant evolutionary relationship with previously isolated viruses and can be grouped into a family-level novel viral cluster (VC_61) with eleven uncultured, assembled viral genomes, named as Psaeviridae. Psaeviridae contains two ORFs (ORFs 117 and 127), which were not detected in the genomes of other viral families, confirming the proposal for a new family. Combined with its ability to infect Pseudomonas and its representation of an unstudied viral family, vB_PsaP_M1 may be an important and novel model system for the study of interactions between viruses and host cells in marine ecosystems.

假单胞菌属（Pseudomonas）是一类广泛分布且分类学上颇具争议的机会致病菌，在海洋生态系统中发挥着重要的生态学功能。本研究报道了一株全新的假单胞菌噬菌体vB_PsaP_M1，该噬菌体分离自中国青岛近岸表层海水。vB_PsaP_M1的基因组为线性双链DNA分子，全长89387 bp，GC含量为41.04%，共编码184个推定开放阅读框（open reading frames, ORFs）。通过BLASTp分析共预测到50个保守结构域，其中包含2个辅助代谢基因：磷酸诱导基因phoH以及信号肽肽酶A（signal peptide peptidase A, sppA）。全基因组氨基酸序列系统发育分析与比较基因组学分析结果显示，vB_PsaP_M1与此前已分离的病毒亲缘关系较远，可与11条未培养的组装病毒基因组共同归为一个科级新型病毒簇（VC_61），并将该病毒簇命名为Psaeviridae科。Psaeviridae科特有的2个开放阅读框（ORF 117与ORF 127）在其他所有已知病毒科的基因组中均未被检测到，这一发现证实了建立该新病毒科的提议。结合其可感染假单胞菌的宿主特性，以及作为一类尚未被研究的病毒科的代表株，vB_PsaP_M1有望成为研究海洋生态系统中病毒与宿主细胞相互作用的重要且新颖的模式系统。