Twin colony isolate genome assemblies: Chlamydomonas 3112/3222 and WS3/WS7 and Scenedesmus ARA/ARA3

Genomes and assemblies for twin isolate pairs from:

David R. Nelson, Amphun Chaiboonchoe, Weiqi Fu, Khaled M. Hazzouri, Ziyuan Huang, Ashish Jaiswal, Sarah Daakour, Alexandra Mystikou, Marc Arnoux, Mehar Sultana, Kourosh Salehi-Ashtiani,Potential for Heightened Sulfur-Metabolic Capacity in Coastal Subtropical Microalgae,iScience,Volume 11,2019,Pages 450-465,ISSN 2589-0042,https://doi.org/10.1016/j.isci.2018.12.035.(https://www.sciencedirect.com/science/article/pii/S2589004218302657)

Abstract: SummaryThe activities of microalgae support nutrient cycling that helps to sustain aquatic and terrestrial ecosystems. Most microalgal species, especially those from the subtropics, are genomically uncharacterized. Here we report the isolation and genomic characterization of 22 microalgal species from subtropical coastal regions belonging to multiple clades and three from temperate areas. Halotolerant strains including Halamphora, Dunaliella, Nannochloris, and Chloroidium comprised the majority of these isolates. The subtropical-based microalgae contained arrays of methyltransferase, pyridine nucleotide-disulfide oxidoreductase, abhydrolase, cystathionine synthase, and small-molecule transporter domains present at high relative abundance. We found that genes for sulfate transport, sulfotransferase, and glutathione S-transferase activities were especially abundant in subtropical, coastal microalgal species and halophytic species in general. Our metabolomics analyses indicate lineage- and habitat-specific sets of biomolecules implicated in niche-specific biological processes. This work effectively expands the collection of available microalgal genomes by ∼50%, and the generated resources provide perspectives for studying halophyte adaptive traits.Keywords: Global Nutrient Cycle; Phycology; Genomics; Metabolomics

https://www.sciencedirect.com/science/article/pii/S2589004218302657

Files are as follows:

.fa = assembly

.tr.fa = coding sequences

.aa.fa = predicted proteins

.gff = annotation files

本数据集为源自以下研究的双分离株对的基因组及其组装序列：
戴维·R·纳尔逊（David R. Nelson）、安蓬·猜本乔伊（Amphun Chaiboonchoe）、傅炜琪（Weiqi Fu）、哈立德·M·哈祖里（Khaled M. Hazzouri）、黄子渊（Ziyuan Huang）、阿什什·杰斯瓦尔（Ashish Jaiswal）、莎拉·达库（Sarah Daakour）、亚历山德拉·米斯蒂库（Alexandra Mystikou）、马克·阿尔努（Marc Arnoux）、梅哈尔·苏丹娜（Mehar Sultana）、库罗什·萨利希-阿什蒂亚尼（Kourosh Salehi-Ashtiani），研究论文题为《亚热带沿海微藻增强硫代谢能力的潜力》，发表于*iScience*，2019年第11卷，第450-465页，ISSN 2589-0042，DOI：10.1016/j.isci.2018.12.035，原文链接：https://doi.org/10.1016/j.isci.2018.12.035（https://www.sciencedirect.com/science/article/pii/S2589004218302657）。

摘要：微藻的代谢活动支撑营养循环，有助于维持水生与陆地生态系统的稳定。绝大多数微藻物种，尤其是亚热带来源的物种，其基因组特征尚未得到解析。本研究报道了22株源自亚热带沿海区域、隶属于多个演化支的微藻物种，以及3株来自温带区域的微藻物种的分离与基因组特征解析工作。这些分离株中绝大多数为耐盐菌株，包括Halamphora属、杜氏藻属（Dunaliella）、拟小球藻属（Nannochloris）以及绿球藻属（Chloroidium）。亚热带来源的微藻中，含有大量富集的甲基转移酶、吡啶核苷酸-二硫键氧化还原酶、α/β水解酶（abhydrolase）、胱硫醚合酶以及小分子转运蛋白结构域。我们发现，硫酸盐转运、磺基转移酶以及谷胱甘肽S-转移酶相关编码基因，在亚热带沿海微藻物种以及一般盐生植物物种中尤为丰富。本研究的代谢组学分析显示，存在一系列演化支与生境特异性的生物分子，参与了生态位特异性的生物学过程。本研究将已公开的微藻基因组数据集扩充了约50%，所生成的研究资源为盐生植物适应性性状的研究提供了新视角。
关键词：全球营养循环；藻类学；基因组学；代谢组学

原文链接：https://www.sciencedirect.com/science/article/pii/S2589004218302657

数据集文件如下：
.fa：基因组组装序列
.tr.fa：编码序列
.aa.fa：预测蛋白序列
.gff：注释文件