Transcriptional analysis of different tylosin high-producing strains.

To investigate gene expression differences of different tylosin high-producing strains, transcriptomes of three tylosin high-producing engineered strains (TLPH08-2, TLPH11 and TLPH17) and the vector control strain TLSET152 were analyzed by RNA-Seq. Different strains (TLSET152, TLPH08-2, TLPH11 and TLPH17) were harvested at 96 h of fermentationat and then RNA isolation, transcriptome sequencing and data analysis were conducted. Comparative gene expression profiling analysis of different genes overexpression strains at 96 h of fermentation .

为探究不同泰乐菌素高产菌株的基因表达差异，本研究采用RNA测序（RNA-Seq）技术，对3株泰乐菌素高产工程菌株（TLPH08-2、TLPH11与TLPH17）及载体对照菌株TLSET152的转录组进行了分析。研究于发酵培养96小时时收集上述4株菌株（TLSET152、TLPH08-2、TLPH11及TLPH17），随后开展RNA提取、转录组测序及数据分析工作，并完成了发酵96小时时不同基因过表达菌株的比较基因表达谱分析。