Ammonia oxidizing community composition of mixed beech forest soils and seepage-mediated translocation in the Hainich Critical Zone Exploratory. Ammonia oxidizer diversity in soil and seepage

We studied the bacterial and archaeal ammonia oxidizing community structure in mixed beech forest soils and the vertically mobilized fraction in seepage in the Hainich region (Thuringia/Germany). Seepage was collected from lysimeter at different depths (litter layer, 0 to 4 cm, 0 to 16 cm, and 0 to 30 cm). Corresponding soil samples at 5 cm and 10 cm depth nearby the lysimeters locations were included. Sequencing of samples was performed four times in 2018 (February, May, July, and November). Community composition was determined by amplicon sequencing using Illumina MiSeq, with the primer combinations amoA-1F/amoA-2R for the bacterial amoA gene and Arch-amoA-104F/Arch-amoA-616R_mod for the archaeal amoA gene.

本研究以德国图林根州海尼希（Hainich）区域的混交山毛榉林土壤，及其渗滤液中的垂直迁移组分为研究对象，解析了其中细菌与古菌氨氧化微生物的群落结构。渗滤液采集自不同深度的渗漏计（lysimeter），采样深度涵盖枯落物层、0~4 cm、0~16 cm与0~30 cm。本研究同时纳入了渗漏计点位周边、深度为5 cm和10 cm的对应土壤样品。样品测序工作于2018年分四次开展，分别为2月、5月、7月及11月。采用Illumina MiSeq平台进行扩增子测序以解析群落组成：针对细菌amoA基因使用引物组合amoA-1F/amoA-2R，针对古菌amoA基因使用引物组合Arch-amoA-104F/Arch-amoA-616R_mod。