Vertebrae recovery of ovariectomy rats using fishbone flour therapy and Calcium Carbonate (CaCO3)

This research was conducted in Ternate City, North Maluku Province. The research has been approved by the Research Ethics Committee with approval No. 220-KEP-UB. This research consists of three stages: first, processing yellowfin fish bone waste into the fishbone meal with deproteinated, non-deproteinized, and calcium carbonate treatments as a comparison. The second research stage was to conduct a physicochemical analysis of the yellowfin fishbone meal. The third stage was using X-rays to confirm the degree of vertebral bone damage in ovariectomy rats treated with deproteinated, non-deproteinated fish bone meal and calcium carbonate. All statistical analyses were performed using SPSS 28.0 software[24]. The differences between groups were compared using Student's t-test, which is to analyses between boiling water and the previous one to determine the difference between acetic acid and hydrochloric acid The results of the chemical analysis carried out on yellowfin fish bone meal include moisture, ash, fat, protein, calcium, and phosphorus content. The results of the chemical analysis show that the highest value of calcium is found in the boiling treatment of yellowfin fish bones using the non-deproteination method, while the high importance for phosphorus is using deproteinated boiling.

本研究于北马鲁古省特尔纳特市开展，已通过研究伦理委员会审批，审批编号为220-KEP-UB。本研究包含三个阶段：第一阶段，将黄鳍金枪鱼鱼骨废料制备为鱼骨粉，设置脱蛋白、未脱蛋白及碳酸钙三组处理作为对照；第二阶段，对黄鳍金枪鱼鱼骨粉开展理化分析；第三阶段，借助X射线检测经脱蛋白鱼骨粉、未脱蛋白鱼骨粉及碳酸钙处理的去卵巢大鼠的椎体骨损伤程度。所有统计分析均采用SPSS 28.0软件[24]完成，组间差异采用学生t检验（Student's t-test）进行比较，该检验用于对比沸水处理组与前期组别，以明确乙酸与盐酸处理间的差异。针对黄鳍金枪鱼鱼骨粉的化学分析指标包括水分、灰分、脂肪、蛋白质、钙及磷含量。分析结果显示，采用未脱蛋白法处理的黄鳍鱼骨粉钙含量最高，而采用脱蛋白沸水处理的样品磷含量最高。