Quantitative Microproteomics Based Characterization of the Central and Peripheral Nervous System of a Mouse Model of Krabbe Disease

In-depth characterization of the proteome of the Twitcher mouse, a mouse model for Krabbe disease, to investigate the proteome changes in the Central and Peripheral Nervous System.We applied a TMT-based workflow to compare the proteomes of the corpus callosum, motor cortex and sciatic nerves of littermate Twitcher and wild-type mice.

本研究针对作为克拉伯病（Krabbe disease）小鼠模型的震颤小鼠（Twitcher mouse）的蛋白质组开展深度表征，以探究其中枢神经系统与外周神经系统内的蛋白质组变化。本研究采用基于串联质量标签（TMT）的实验流程，对同窝震颤小鼠与野生型小鼠的胼胝体、运动皮层及坐骨神经的蛋白质组进行比较分析。