Interstrain Variation in the Human Cytomegalovirus DNA Polymerase Sequence and Its Effect on Genotypic Diagnosis of Antiviral Drug Resistance

The polymerase (pol) coding sequence was determined for 40 independent clinical cytomegalovirus isolates sensitive to ganciclovir and foscarnet. Sequence alignments showed >98% interstrain homology and amino acid variation in only 4% of the 1,237 codons. Almost all variation occurred outside of conserved functional domains where resistance mutations have been identified.

本研究对40株临床独立分离的、对更昔洛韦（ganciclovir）和膦甲酸钠（foscarnet）敏感的巨细胞病毒（cytomegalovirus）分离株，测定了其聚合酶（polymerase, pol）编码序列。序列比对结果显示，株间同源性超过98%，且在1237个密码子中仅4%存在氨基酸变异。几乎所有变异均发生在已报道存在耐药突变的保守功能结构域之外。