Epigenetic landscape of Human Brains by Single Nucleus DNA Methylation and Chromatin Conformation Profiling - pool56_h1930001_MEC

Illustrating the cellular architecture of the human brain is critical to understanding its diverse functions and complex animal behaviors. Single nucleus methylation sequencing was applied to regions of human brains. we identified distinct cell clusters and formed them in a hierarchical taxonomy. These clusters include known primary brain cell types and possible sub-types. We use these data to identify the epigenomic characteristics and to define specific regulatory elements for each cell cluster. Overall design: Apply snmC-seq3 to brain regions to identify epigenomic landcape of the adult human brains

阐明人脑的细胞架构，对于理解其多样功能与复杂的动物行为至关重要。本研究对人脑多个区域实施单细胞核甲基化测序（Single nucleus methylation sequencing），识别出不同的细胞簇并构建了层级分类体系。这些细胞簇涵盖了已被报道的主要脑细胞类型及潜在亚型。本研究利用上述数据，解析了各细胞簇的表观基因组特征，并明确了其特异性调控元件。整体实验设计：采用单细胞核甲基化测序3（snmC-seq3）对大脑区域进行测序，以解析成人脑的表观基因组全景。