Effect of SLC25A1 knockdown on gene expression during differentiation of human airway basal cells. Effect of SLC25A1 knockdown on gene expression during differentiation of human airway basal cells

To investigate the role of mitochondrial citrate export in differentiation of airway basal cells, we knocked down SLC25A1, the gene encoding mitochondrial citrate carrier. Overall design: We then performed gene expression profiling analysis using data obtained from RNA-seq of 5 donors at three time points. Comparative gene expression profiling analysis of RNA-seq data for human airway basal cells and its SLC25A1 knockdown derivative (shSLC25A1)

为探究线粒体柠檬酸输出在气道基底细胞分化中的作用，我们敲低了编码线粒体柠檬酸载体的基因SLC25A1。实验整体设计：我们基于5名供体在三个时间点获取的RNA测序（RNA-seq）数据开展了基因表达谱分析；此外，还针对人气道基底细胞及其SLC25A1敲低衍生物（shSLC25A1）的RNA测序数据进行了对比基因表达谱分析。