Microbiological diversity analysis data of animal manure and biogas slurry from Guangxi , China

Harmless treatment and resource utilization of livestock manure are the ultimate concerns of agronomists and environmentalists. A treatment of mesophilic anaerobic fermentation was the wide method to disposal livestock manure, and there are many crafts with different parameters. And now, there are few reports explained the influence of bacteria and pathogens after treated by mesophilic anaerobic fermentation. In this study, we used high-throughput sequencing technology to analysis the composition and diversity of bacteria and pathogens in fecal samples and digestates under three methods of mesophilic anaerobic fermentation. According to the results, three ways of anaerobic fermentation all can change the composition and diversity of bacteria and pathogens, and the traditional mesophilic anaerobic fermentation had the least effect on the composition and diversity of bacteria and pathogens in feces, while the mesophilic anaerobic treatment with extra bacteria had the greatest effect. This study contributes to the safe and rational use of digestates and help to update existing mesophilic anaerobic fermentation to improve the ability of remove pathogens.

畜禽粪便的无害化处理与资源化利用，始终是农学家与环境学家关注的核心议题。中温厌氧发酵（mesophilic anaerobic fermentation）曾是畜禽粪便处置的主流工艺，且存在诸多参数各异的技术方案。然而目前鲜有研究阐明中温厌氧发酵处理后菌群与病原菌的变化规律。本研究采用高通量测序（high-throughput sequencing）技术，分析了三种中温厌氧发酵工艺处理前后粪便样品及其发酵产物沼液（digestate）中的菌群与病原菌组成及多样性特征。研究结果显示，三种厌氧发酵工艺均能改变粪便中菌群与病原菌的组成及多样性；其中传统中温厌氧发酵对粪便菌群与病原菌组成及多样性的影响最弱，而添加外源菌剂的中温厌氧处理则影响最为显著。本研究可为沼液的安全合理利用提供理论支撑，同时有助于优化现有中温厌氧发酵工艺，提升其对病原菌的去除效能。