RNA-seq Reveals Macrophage Polarization and Fibrosis Markers in AKI to CKD Transition After Ischemia-Reperfusion Injury

RNA-seq analysis of kidney samples from mice after ischemia-reperfusion injury (IRI) identified 1,109 genes with altered expression between mild (20 minutes) and severe (35 minutes) IRI groups. Among these, 974 genes were upregulated and 135 were downregulated in the severe group. Overall design: The experimental design involved using C57Bl/6 mice to study the transition from acute kidney injury (AKI) to chronic kidney disease (CKD) following ischemia-reperfusion injury (IRI). Two groups were established based on the duration of ischemia: a mild IRI group with 20 minutes of ischemia and a severe IRI group with 35 minutes of ischemia. The mice were sacrificed 28 days post-IRI to assess the long-term effects of the injury.

针对缺血再灌注损伤（ischemia-reperfusion injury, IRI）后小鼠肾脏样本的RNA测序（RNA-seq）分析，在轻度（20分钟）与重度（35分钟）IRI组间鉴定出1109个差异表达基因。其中，重度组中974个基因表达上调，135个基因表达下调。整体实验设计：本实验以C57Bl/6小鼠为研究对象，旨在探究缺血再灌注损伤后急性肾损伤（acute kidney injury, AKI）向慢性肾病（chronic kidney disease, CKD）的转归进程。实验根据缺血时长设置两组：缺血20分钟的轻度IRI组，以及缺血35分钟的重度IRI组。所有小鼠均于IRI造模后28天处死，以评估损伤的长期效应。