Identification of the covalent binding site of Ibrutinib and 5Z-7-Oxozeaenol to ZAK

The goal of this experiment was to identify the reaction site of Ibrutinib and 5Z-7-Oxozeaenol to ZAK by determination of mass shifts according to a covalent modification by the inhibitors using mass spectrometry.

本实验旨在通过质谱技术检测抑制剂共价修饰引发的质量位移，以此鉴定依鲁替尼（Ibrutinib）与5Z-7-氧代泽兰醇（5Z-7-Oxozeaenol）同ZAK的结合反应位点。