Genomic Glucocorticoid Action in Embryonic Mouse Neural Stem Cells

The goal of this study was to measure genome-wide expression in primary mouse neural stem/progenitor cell (NSPC) cultures to determine if SOX2 ablation alters the transcriptomic response which occurs following glucocorticoid receptor activation by the synthetic glucocorticoid, Dexamethasone. Neurosphere cultures of SOX2 knock out (KO) NSPCs and control non-deleted wild-type (WT) NSPCs (C57BL/6) derived from the fetal telencephalon were established at postnatal day zero (P0). After the third passage, biologically distinct replicates of both sexes were treated in-vitro with 100nM Dexamethasone (Dex) for 4 hours and processed for microarray gene expression analysis (n=6 WT; n=7 SOX2 KO).

本研究旨在对原代小鼠神经干细胞/前体细胞（neural stem/progenitor cell, NSPC）培养物进行全基因组表达量检测，以明确SOX2基因敲除是否会改变合成糖皮质激素地塞米松（Dexamethasone）激活糖皮质激素受体后所诱导的转录组应答。本研究从出生后第0天（P0）的小鼠胎儿端脑中分离得到SOX2敲除（KO）型NSPC与未敲除的野生型（WT）C57BL/6小鼠NSPC，并建立了两类细胞的神经球培养体系。传至第3代后，对雌雄两性的独立生物学重复样本以100纳摩尔浓度的地塞米松（Dex）进行体外处理4小时，随后开展基因芯片表达分析（野生型组n=6；SOX2敲除组n=7）。