Table_1_Characterization of Lactobacillus rhamnosus MP01 and Lactobacillus plantarum MP02 and Assessment of Their Potential for the Prevention of Gastrointestinal Infections in an Experimental Canine Model.DOCX

The aim of the present study was to evaluate the probiotic potential of Lactobacillus rhamnosus MP01 and Lactobacillus plantarum MP02, two strains isolated from canine milk. These two isolates were characterized in vitro for their survival to conditions similar to those found in the canine gastrointestinal tract, production of antimicrobial compounds, adherence to intestinal mucin, degradation of mucin, and antibiotic sensitivity. Globally, both strains exhibited a high in vitro probiotic potential. Finally, their potential for the prevention of gastrointestinal infections was evaluated in an experimental canine model using 1-month-old puppies. A group of 12 German shepherd puppies, 6 males and 6 females, received L. rhamnosus MP01 daily for 2 months and a second group of 12 puppies, 6 males and 6 females, of the same breed received L. plantarum MP02 during the same period of time. The same experimental approach was carried with Yorkshire puppies. Additionally, the trial included 12 dogs of each breed in the placebo groups. The results demonstrated that administration of the strains resulted in a significant preventive effect of gastrointestinal infections in such populations.

本研究旨在评估从犬乳汁中分离得到的两株乳酸菌——鼠李糖乳杆菌（Lactobacillus rhamnosus）MP01与植物乳杆菌（Lactobacillus plantarum）MP02的益生潜能。本研究通过体外实验对两株分离菌进行了系统表征，检测指标涵盖其在模拟犬胃肠道环境下的存活率、抗菌化合物生成能力、肠道黏附能力、黏蛋白降解能力以及抗生素敏感性。整体而言，两株菌株均表现出优异的体外益生潜能。随后，本研究以1月龄幼犬为实验对象构建犬类感染模型，评估两株菌株预防胃肠道感染的潜力：将12头德国牧羊犬幼犬（6公6母）设为鼠李糖乳杆菌MP01实验组，每日灌服该菌株，持续2个月；另设12头同品种幼犬（6公6母）作为植物乳杆菌MP02实验组，同期灌服该菌株。本实验同时以约克夏幼犬重复了上述实验流程。此外，本试验还为每个品种设置了12头犬的安慰剂对照组。实验结果表明，灌服上述菌株可对该类群体产生显著的胃肠道感染预防效果。