De novo genome assemblies of lemon selected and wild-type selected mites from a segregating T. kanzawai population and its lemon and wild-type parent.

The segregating mite population was generated by crossing Jp-inbred-lemon to Jp2-WT and was used to genetically map the lemon phenotype in Tetranychus kanzawai. After approximately 10-12 generations, a total of three replicates were collected that consisted of 1100, 900 and 500 adult lemon females. In parallel, 1500 wild-type females were selected and pooled into a single sample. From these four populations and the two parental strains, genomic DNA was extracted and assembled using the CLC Genomics Workbench 9.0.1 (https://www.qiagenbioinformatics.com/).

本研究所用螨类分离群体通过将Jp-inbred-lemon与Jp2-WT杂交构建，用于对神泽叶螨（Tetranychus kanzawai）的柠檬色表型开展遗传作图分析。经约10-12代繁育后，共收集3个生物学重复样本，其所含柠檬色雌成螨数量分别为1100头、900头与500头。同时，选取1500头野生型雌成螨并混合为单一样本。从上述4个群体及2个亲本品系中提取基因组DNA，并使用CLC Genomics Workbench 9.0.1（https://www.qiagenbioinformatics.com/）完成基因组组装。