Gene expression profiles of healthy mononuclear cells and eosinophils and asthma (moderate and severe) eosinophils

We used scRNAseq to better characterize human eosinophils at resting state in healthy patients (compared to other MNCs). We then compared the healthy eosinophils to eosinophils from asthmatic patients (moderate and severe). Overall design: Sample preparation was optimized to isolate and process human eosinophils, followed by analysis by scRNAseq.

本研究采用单细胞RNA测序（scRNAseq）技术，对健康受试者静息状态下的人类嗜酸性粒细胞（相较于其他单个核细胞（MNCs））开展更为精准的特征表征。随后将健康受试者体内的嗜酸性粒细胞，与中度及重度哮喘患者来源的嗜酸性粒细胞进行对比分析。总体实验设计：样本制备流程经过优化，以分离并处理人类嗜酸性粒细胞，随后通过scRNAseq完成测序分析。