RNA Sequencing of two Breast Cancer Cell Lines in Comparison to Healthy Epithelial Breast Cells

We studied genes, that are differentially expressed between malignant and normal breast tissue, to find weak spots for anti-cancer therapy development. RNA sequencing of three cell lines was performed: MCF-7 (epithelial breast cancer cell line), BCC (primary breast tumour cell line) and MCF-10A (epithelial breast cell line). Overall design: Three biological replicates were analyzed for each of 3 studied cell lines

本研究针对恶性乳腺组织与正常乳腺组织间存在差异表达的基因开展分析，旨在发掘抗癌疗法开发的潜在干预靶点。本研究对3种细胞系进行了RNA测序，分别为MCF-7（上皮性乳腺癌细胞系）、BCC（原发性乳腺肿瘤细胞系）以及MCF-10A（上皮性乳腺细胞系）。整体实验设计：3种受试细胞系各设置3次生物学重复，并完成了相关测序分析。