Methylation studies in plains zebras. Methylation studies in plains zebras

An Infinium microarray platform (GPL28271, HorvathMammalMethylChip40) was used to generate DNA methylation data from many tissues of plains zebras (Equus quagga). Both whole blood (96) and remote biopsy (24) samples were obtained from a captive population of zebras maintained in a semi-wild state. After eliminating samples with low confidence for individual identity and age, we retained 76 blood samples and 20 biopsy samples, totaling 96 zebra samples. Overall design: Genome annotations of the mammalian methylation array can be downloaded from https://github.com/shorvath/MammalianMethylationConsortium

本研究采用Infinium微阵列平台（GPL28271，HorvathMammalMethylChip40），对平原斑马（Equus quagga）的多种组织开展DNA甲基化数据检测。实验样本取自处于半野生状态的圈养斑马种群，包含全血样本96份与远程活检样本24份。在剔除个体身份与年龄置信度较低的样本后，最终保留全血样本76份、活检样本20份，总计96份斑马样本。实验总体设计如下：哺乳动物甲基化芯片的基因组注释信息可从https://github.com/shorvath/MammalianMethylationConsortium 下载。