Hevea brasiliensis subsp. brasiliensis strain:clone RRIM 600 Transcriptome or Gene expression

Hevea brasiliensis is the major economic crop as a source of natural rubber production. However, limited information of genomic resource and the lack of molecular markers developed for this plant species hinder breeding programs for crop improvement. To gain a better understanding of the rubber tree genome, we generated Expressed Sequence Tags (ESTs) and developed microsatellite markers from the transcriptome of H. brasiliensis leaf. We have sequenced 2,265,782 reads from the using 454 pyrosequencing technology. After clustering and assembly, a total of 214,013 unique sequences, comprising 32,633 contigs and 181,380 singlets, were generated. Gene annotation revealed that 49% shared sequence similarity with proteins in the Genbank non-redundant protein database. Functional categorization was performed by mapping against Gene Ontology (GO). We also identified 43,865 loci microsatellites from the data set.

巴西橡胶树（Hevea brasiliensis）是天然橡胶生产的核心经济作物。然而，该物种的基因组资源信息有限，且缺乏已开发的分子标记，这极大阻碍了其作物改良的育种工作。为深入解析橡胶树的基因组特征，本研究从巴西橡胶树叶片的转录组中生成了表达序列标签（Expressed Sequence Tags, ESTs）并开发了微卫星标记（microsatellite markers）。我们采用454焦磷酸测序技术（454 pyrosequencing）共获得2,265,782条读长序列。经聚类与组装后，最终得到214,013条非冗余序列，其中包含32,633个重叠群（contigs）与181,380个单序列（singlets）。基因注释结果显示，49%的序列与GenBank非冗余蛋白数据库中的蛋白存在序列相似性。本研究通过比对基因本体（Gene Ontology, GO）数据库完成了功能分类注释，并从该数据集中共鉴定出43,865个微卫星位点。