Impact of dioxin exposure and high fat diet feeding on adult mouse islets. Impact of dioxin exposure and high fat diet feeding on adult mouse islets

Purpose: to determine whether chronic low dose dioxin exposure in adult male and female mice impacts susceptibility to metabolic disease and islet dysfunction. Overall design: Male and female C57Bl/6 mice were injected i.p. 2x/week with either corn oil or 20 ng/kg/d TCDD (2,3,7,8-tetrachlorodibenzo-p-dioxin). Each treatment group was further subdivided to receive either chow diet or 45% kcal high fat diet (HFD). Pancreatic islets were isolated 12 weeks after the beginning of treatment.

研究目的：明确成年雌雄C57Bl/6小鼠长期低剂量二噁英暴露是否会影响其代谢疾病易感性与胰岛功能障碍。实验设计：对雌雄C57Bl/6小鼠每周腹腔注射（intraperitoneal，简称i.p.）两次，分别给予玉米油或20 ng/kg/d的2,3,7,8-四氯二苯并对二噁英（TCDD）。每个处理组进一步细分为普通饲料组与45%热量占比的高脂饮食（HFD）组。于干预开始12周后分离胰腺胰岛。