Transcriptome profiling of guinea pig cytomegalovirus infected cells

We report the transcriptome profiles of guinea pig cytomegalovirus (Caviid betaherpesvirus 2 ; GPCMV) infected and uninfected cells. RNA was harvested at an early time post-infection. Data sets from a lung fibroblast cell line (JH4), primary amnion derived cells (1° AECs), and HPV16 E6/E7-transduced amniotic epithelial cells (AECD) is shared. Overall design: Confluent guinea pig lung fibroblasts (JH4 clone 1), primary guinea pig amnion derived cells (1° AECs), or HPV16 E6/E7-transduced amniotic epithelial cells (AECD) were infected with guinea pig cytomegalovirus (GPCMV, Caviid betaherpesvirus 2) or mock infected in triplicate. RNA was extracted from these cells at 24 hours post-infection and sequenced by Illumina RNA-Seq. Data represents three expreiments and eightteen total samples.

本研究报道了感染与未感染豚鼠巨细胞病毒（guinea pig cytomegalovirus，分类学命名为Caviid β疱疹病毒2型，简称GPCMV）的细胞的转录组图谱。实验于感染后早期时段收集RNA样本，本次共享的数据集涵盖肺成纤维细胞系（JH4）、原代羊膜来源细胞（1° AECs）以及经人乳头瘤病毒16型E6/E7（HPV16 E6/E7）转导的羊膜上皮细胞（AECD）的测序数据。
实验设计概述：将汇合状态的豚鼠肺成纤维细胞（JH4克隆1）、原代豚鼠羊膜来源细胞（1° AECs）以及经人乳头瘤病毒16型E6/E7（HPV16 E6/E7）转导的羊膜上皮细胞（AECD）分别以豚鼠巨细胞病毒（GPCMV，Caviid β疱疹病毒2型）进行感染，同时设置假感染（Mock感染）对照组，每组均设置3个生物学重复。于感染后24小时收集上述细胞并提取RNA，采用Illumina RNA-Seq技术完成测序。本数据集共包含3次独立实验的总计18个样本的测序结果。