Regulation of host RNA splicing in Mycobacterium tuberculosis infected macrophages. Regulation of host RNA splicing in Mycobacterium tuberculosis infected macrophages

Here using a high-throughput yeast two-hybrid and mass spectrometry approach we show that mycobacterial proteins can access the RNA splicing machinery of the host, thereby playing a critical role in infection-induced perturbation of alternative splicing. Overall design: We then performed gene expression profiling analysis using data obtained from RNA-seq of THP-1 macrophages infected with H37Rv and H37Rv::Rv1435KO at 48hr post infection.

本研究借助高通量酵母双杂交（yeast two-hybrid）与质谱（mass spectrometry）技术，证实分枝杆菌蛋白可靶向宿主的RNA剪接机器（RNA splicing machinery），进而在感染诱导的可变剪接（alternative splicing）紊乱中发挥关键调控作用。总体实验设计：我们对感染H37Rv菌株及H37Rv::Rv1435KO基因敲除株的THP-1巨噬细胞，在感染后48小时开展RNA-seq测序，并基于所得数据进行基因表达谱分析。