Id1 and Id3 Maintain Steady State Hematopoiesis by Promoting Sinusoidal Endothelial Cell Survival and Regeneration. Id1 and Id3 Maintain Steady State Hematopoiesis by Promoting Sinusoidal Endothelial Cell Survival and Regeneration

Single cell RNA-Seq was performed on ID1 ID3 null endothelial cells to determine the effects of ID gene loss on endothelial cell maintainence and growth. Overall design: 20,000 SECs and type H vessels from CDH5-CreER ID1+/+ ID3+/- or CDH5-CreER ID1-/- ID3-/- mice were sorted using FACs and subjected to single cell RNA-Seq.

本研究针对ID1、ID3双功能缺失内皮细胞开展单细胞RNA测序（Single cell RNA-Seq），旨在明确ID基因缺失对内皮细胞稳态维持与增殖的影响。
实验设计：从携带CDH5-CreER重组酶系统、基因型分别为ID1+/+ ID3+/- 与ID1-/- ID3-/-的小鼠中，分选出20000个窦状内皮细胞（SECs）及H型血管，经荧光激活细胞分选（Fluorescence-Activated Cell Sorting, FACs）处理后，对上述样本进行单细胞RNA测序。