Ambrosia trifida Transcriptome or Gene expression

Giant ragweed (Ambrosia trifida) is one of the most competitive annual weeds in corn and soybean production across the eastern Corn Belt in the United States. The use of glyphosate (commercial name: Roundup) and glyphosate-ready crop systems managed to keep giant ragweed population under control for many years. Glyphosate-ready crop systems are genetically resistant to glyphosate, which enables farmers to use glyphosate to control the population of weeds. But in the last decade, glyphosate-resistant giant ragweed has been reported. The transcriptome was sequenced by extracting the total mRNA from leaf disks and performing RNA-Seq. The sequences were assembled using Trinity. Trinotate was then used for annotation. The giant ragweed transcriptome contained 61,000 genes and was found to be 97% complete. The transciptome was sequenced and annotated with the aim of helping understand the mechanisms of resistance to glyphosate.

三裂叶豚草（Ambrosia trifida）是美国东部玉米带内玉米与大豆种植体系中竞争力最强的一年生杂草之一。草甘膦（glyphosate，商品名：农达（Roundup））与抗草甘膦作物体系曾在多年间有效控制三裂叶豚草种群规模。抗草甘膦作物体系经基因改造对草甘膦具有抗性，可使种植者通过施用草甘膦防控杂草种群。但近十年来，已有抗草甘膦三裂叶豚草的相关报道。本研究通过从叶盘样本中提取总mRNA并开展RNA测序（RNA-Seq）获得转录组序列，使用Trinity软件完成序列组装，随后通过Trinotate工具进行功能注释。该三裂叶豚草转录组共包含61000个基因，序列完整性达97%。本次转录组测序与注释工作旨在助力解析三裂叶豚草的草甘膦抗性机制。