Additional file 1 of Comparative analysis of hippocampal extracellular space uncovers widely altered peptidome upon epileptic seizure in urethane-anaesthetized rats

Additional file 1: Table S1. List of detected peptides in microdialysate samples. Peptide sequences, positions, Uniprot accession numbers, protein names, and normalized abundances are shown. (Norm ab: normalized abundance; 4Ap: 4-AP treated samples; ctrl: control samples). Table S2. List of significantly altered peptides upon 4-AP treatment (paired t-test, adjusted P-value < 0.05). Peptide sequences, positions, Uniprot accession numbers, gene symbols, and protein names are shown. Table S3. List of peptides that are detected in at least 9 of the 4-AP treated samples and not more than 2 of the control samples upon 4-AP treatment. Peptide sequences, positions, modifications, Uniprot accession numbers, gene symbols, and normalized abundances (Norm ab) are shown. (4Ap: 4-AP treated samples; ctrl: control samples). Table S4. Enrichment analysis on upregulated proteins. (Analysis was carried out by g:Profiler (version e106_eg53_p16_65fcd97) g:GOSt online tool with the background of Rattus norvegicus genome). Table S5. Enrichment analysis on downregulated proteins. (Analysis was carried out by g:Profiler (version e106_eg53_p16_65fcd97) g:GOSt online tool with the background of Rattus norvegicus genome). Table S6. Functional categories of proteins that had significantly altered peptides in the microdialysate.

附加文件1：表S1。微透析样品中检测到的肽段列表。包含肽段序列、检测位点、UniProt（通用蛋白质数据库）登录号、蛋白质名称及归一化丰度信息。（Norm ab：归一化丰度；4Ap：4-氨基吡啶（4-AP）处理样品；ctrl：对照样品）。
表S2。经4-氨基吡啶（4-AP）处理后发生显著变化的肽段列表（配对t检验，校正后P值＜0.05）。包含肽段序列、检测位点、UniProt登录号、基因符号及蛋白质名称信息。
表S3。满足以下条件的肽段列表：在4-氨基吡啶处理样品中至少9次被检测到，且在对照样品中被检测到的次数不超过2次。包含肽段序列、检测位点、修饰信息、UniProt登录号、基因符号及归一化丰度（Norm ab）信息。（4Ap：4-氨基吡啶处理样品；ctrl：对照样品）。
表S4。上调蛋白质的富集分析结果。本次分析由g:Profiler（版本e106_eg53_p16_65fcd97）的g:GOSt在线工具完成，以褐家鼠（Rattus norvegicus）基因组作为背景数据集。
表S5。下调蛋白质的富集分析结果。本次分析由g:Profiler（版本e106_eg53_p16_65fcd97）的g:GOSt在线工具完成，以褐家鼠基因组作为背景数据集。
表S6。微透析样品中携带显著变化肽段的蛋白质的功能分类。