SAIMOS - Biological and Flow Cytometry data collected from sampling stations in South Australia, in August 2021

Flow cytometry data was collected in August 2021, in waters off South Australia, from the Upper Spencer Gulf (SAMUSG). The general purpose of the study is to be able to establish background knowledge on the ecosystem on the continental shelf of South Australia and the impact of upwelling/saline outflow events on microbial communities to ultimately develop a biogeochemical model of the region. Sampling was carried out during cruises conducted on board the RV Ngerin as part of the Southern Australian Integrated Marine System (SAIMOS). During each cruise, the physical, chemical and biological properties of the chlorophyll fluorescence maximum (DCM) layer were investigated. Flow cytometry data has been collected for picophytoplankton, bacteria and viruses.Six main stations have been sampled over the course of the study, five are located on the 100 m isobath, i.e. RS (35.508S, 136.278E), B2 (35.418S, 136.148E), B3 (35.258S, 136.048E), SAM2CP/B4 (35.168S, 135.418E) and SAM5CB/B5 (35.008S, 135.198E), and one from an offshore station (B1; 36.188S, 136.178E) located southwest of Kangaroo Island. Note that combining the distances between stations (14–25 nautical miles), the average component of the current velocity at middepth along the shelf (0.01 m s21) and the average speed of the vessel (i.e. 9 knots) indicate that different water masses were sampled at each station. Additional samples have on occasion been collected from the National Reference Station (NRS) at Kangaroo Island (35.832S, 136.447E) and the SA Spencer Gulf Mouth Mooring (SAM8SG, 35.25S, 136.690E), where the saline outflow occurs.Since July 2019 two additional BGC stations have been added to the SAIMOS sampling array: the SA Upper Spencer Gulf Mooring (SAMUSG, 33.111S, 137.709E) and the SA Gulf St Vincent Mooring (SAMGSV, 35.023S, 138.46E).

流式细胞术（Flow cytometry）数据于2021年8月在南澳大利亚近海水域的上斯潘塞湾（Upper Spencer Gulf，SAMUSG）采集。

本研究的总体目标是建立南澳大利亚大陆架生态系统的背景知识，以及上升流/盐流出事件对微生物群落的影响，最终开发该区域的生物地球化学模型（biogeochemical model）。采样工作在恩格林号考察船（RV Ngerin）执行的航次中进行，属于南澳大利亚综合海洋系统（Southern Australian Integrated Marine System，SAIMOS）的一部分。每次航次均调查叶绿素荧光最大值（chlorophyll fluorescence maximum，DCM）层的物理、化学和生物特性。研究采集了微微型浮游植物（picophytoplankton）、细菌和病毒的流式细胞术数据。

研究期间共对6个主要站点进行采样，其中5个位于100米等深线（isobath）处，即RS（35.508°S，136.278°E）、B2（35.418°S，136.148°E）、B3（35.258°S，136.048°E）、SAM2CP/B4（35.168°S，135.418°E）和SAM5CB/B5（35.008°S，135.198°E）；另有1个离岸站点（B1；36.188°S，136.178°E）位于袋鼠岛西南部。需注意，结合站点间距离（14–25海里）、陆架中层平均流速分量（0.01 m/s）及船舶平均航速（即9节）可知，每个站点采集的水体属于不同水团。此外，研究偶尔还从袋鼠岛的国家参考站（National Reference Station，NRS）（35.832°S，136.447°E）和南澳大利亚斯潘塞湾口锚系站（SAM8SG，35.25°S，136.690°E）采集样本，后者为盐流出发生地。

自2019年7月起，SAIMOS采样阵列新增了两个生物地球化学站（BGC，biogeochemical）：南澳大利亚上斯潘塞湾锚系站（SAMUSG，33.111°S，137.709°E）和南澳大利亚圣文森特湾锚系站（SAMGSV，35.023°S，138.46°E）。