Global lncRNA/mRNA expression analysis of colorectal tumor and non-tumor samples from 6 colorectal cancer patients

Given the role of lncRNAs and mRNAs in tumor pathogenesis, we set out to identify transcripts that potentially drive colorectal tumorigenesis. To do this, we performed microarray analysis on matched colorectal tumor and non-tumor samples from 6 colorectal cancer patients, and determined their lncRNA and mRNA expression profiles with Agilent microarrays (Arraystar Human LncRNA Array v3.0). Fresh colorectal tumor samples and matched non-tumor samples (obtained from unaffected colorectal at least 5 cm from the tumor) from 6 colorectal cancer patients were selected randomly for microarray analysis.

鉴于长链非编码RNA（long non-coding RNA, lncRNA）与信使RNA（messenger RNA, mRNA）在肿瘤发生机制中的作用，本研究旨在鉴定潜在驱动结直肠癌发生的转录本。为实现这一研究目标，我们对6名结直肠癌患者的配对结直肠癌肿瘤组织与正常组织样本开展了微阵列分析，并采用Agilent微阵列（Arraystar Human LncRNA Array v3.0）测定了样本的lncRNA与mRNA表达谱。本次研究所用样本为随机选取的6名结直肠癌患者的新鲜结直肠癌肿瘤组织，以及取自距离肿瘤至少5厘米的未受累结直肠组织的配对正常组织。