Whole cell proteomics analysis of Trypanosoma brucei 75kDa invariant surface glycoprotein knockout cells

A superfamily of invariant surface glycoproteins (ISGs) populates the African trypanosome surface, one of which, ISG75, is implicated in uptake of the century-old drug suramin. We disrupted the ISG75 gene locus encoding an array of six ISG75 paralogs on chromosome V by CRISPR/Cas9 knockout. Here we characterise this knockout strain by quantitative whole cell proteomics. Additionally, we analyse the way ISG75 knockout cells respond to suramin on the proteome level.

一类恒定表面糖蛋白（invariant surface glycoproteins, ISGs）超家族定位于非洲锥虫的细胞表面，其中ISG75这一成员与已有百年历史的药物苏拉明（suramin）的摄取过程密切相关。我们借助CRISPR/Cas9基因敲除技术，破坏了五号染色体上编码六个ISG75旁系同源基因簇的ISG75基因座。本研究通过定量全细胞蛋白质组学对该敲除菌株进行了系统表征，并从蛋白质组层面解析了ISG75敲除细胞对苏拉明的应答模式。