The complete genome sequence of the ghost bat, <i>Macroderma gigas</i>

The ghost bat (<i>Macroderma gigas)</i> is a large carnivorous bat inhabiting monsoonal tropics and arid habitat across northern Australia and is currently listed as Vulnerable on the IUCN Red List. We sequenced long-reads of DNA using PacBio HiFi to generate a 2.00 Gb genome consisting of 313 scaffolds, and accompany this with short-read transcriptomes from five tissues to aid in genome annotation using Fgenesh++. The generation of a reference genome will assist in conservation of the species, particularly in relation to understanding the genomic basis of arid adaptation and ongoing development of non-invasive genetic monitoring techniques.

幽灵蝙蝠（Macroderma gigas）是一种大型食肉蝙蝠，栖息于澳大利亚北部的季雨林热带区域与干旱生境，目前已被列入《世界自然保护联盟濒危物种红色名录》（IUCN Red List）的易危等级。本研究采用PacBio HiFi技术对其DNA进行长读长测序，构建了包含313个支架（scaffold）、总长度达2.00 Gb的基因组；同时辅以5种组织的短读长转录组数据，以借助Fgenesh++工具辅助基因组注释。该参考基因组的构建将有助于该物种的保护工作，尤其可为解析干旱适应的基因组基础，以及持续开发非侵入式遗传监测技术提供支撑。