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Quantseq 3'mRNA-seq in LESCs from WT and TXNIP-/- mice

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RNA samples were collected from LSECs isolated from TXNIP-/- and WT mice. High-throughput sequencing was performed as single-end 75 sequencing using NextSeq 550. High-throughput sequencing was performed as single-end 75 sequencing using NextSeq 550 in LSECs isolated from TXNIP-/- and WT mice

研究人员从硫氧还蛋白相互作用蛋白基因敲除(TXNIP-/-)与野生型(WT)小鼠的肝窦内皮细胞(Liver Sinusoidal Endothelial Cells,LSECs)中提取了RNA样本。采用NextSeq 550测序平台进行单端75碱基高通量测序;针对上述分离得到的LSECs,同样使用NextSeq 550平台开展单端75碱基高通量测序。

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