The transcription factor Gli3 promotes differentiation from double positive to CD4 single positive thymocyte by repression of Shh. Mus musculus

We used Affymetrix microarrays to understand the genome wide differences in Wildtype and Gli3 mutant (Gli3+/- and Gli3-/-) (n=2) embryonic day 18.5 DP CD69-, DP CD69+ and SP4 thymocytes. Overall design: Wildtype and Gli3 mutant (Gli3+/- and Gli3-/-) (n=2) embryonic day 18.5 fetal thymus were sorted for DP69- (CD4+CD8+CD69-), DP69+ (CD4+CD8+CD69+) and SP4 (CD4+CD8-) cells. RNA extracted from these cells was sequenced to help understand the transcriptional changes governing DP to SP thymocyte differentiation and selection in the Gli3 mutant.

我们采用Affymetrix微阵列（Affymetrix microarrays）技术，旨在解析野生型与Gli3突变体（包含Gli3+/-和Gli3-/-两种基因型，每组样本量n=2）的胚胎第18.5天DP CD69⁻、DP CD69⁺及SP4胸腺细胞的全基因组表达差异。整体实验设计：本研究从野生型及Gli3突变体（Gli3+/-、Gli3-/-，每组样本量n=2）的胚胎第18.5天胎儿胸腺中，分选得到DP69⁻（CD4⁺CD8⁺CD69⁻）、DP69⁺（CD4⁺CD8⁺CD69⁺）与SP4（CD4⁺CD8⁻）细胞；提取上述细胞的总RNA并进行测序，以阐明调控Gli3突变体中DP胸腺细胞向SP胸腺细胞分化与选择过程的转录组变化。