Organic osmolyte betaine mitigates the deleterious effects of Diclofenac in vivo in wistar albino rats

Abstract Diclofenac sodium (DF) is a non-steroidal anti-inflammatory drug (NSAID) that possesses antipyretic, analgesic, antinociceptive and anti-inflammatory activities. Like other NSAIDs, DF is known to be associated with renal, cardiovascular, and gastrointestinal complications. The present study was carried out to evaluate the adverse effects of DF in vivo in wistar albino rats and to assess if oral administration of the organic osmolyte betaine mitigates the adverse effect of DF. Eighteen male Wistar rats were divided into three groups, one group of animals was fed orally with 20 mg/kg of DF once/day, and the other group received a combination of 20 mg/kg of DF and 30 mg/kg of betaine, once/day. Apart from the hematological and biochemical parameters, histopathological changes in the liver, lungs, brain, heart and kidney were also investigated. Histopathological alterations that were found in the liver, kidney, and lungs of DF-treated animals were found to be minimal or absent in DF + betaine-treated animals, as compared to untreated control. The results showed that betaine mitigates the adverse effects associated with DF treatment.

摘要 双氯芬酸钠（Diclofenac sodium, DF）属于非甾体抗炎药（non-steroidal anti-inflammatory drug, NSAID），具有解热、镇痛、抗伤害感受及抗炎活性。与其他非甾体抗炎药类似，双氯芬酸钠已知可引发肾、心血管及胃肠道并发症。本研究旨在评估双氯芬酸钠对Wistar白化大鼠的体内不良反应，并探究口服有机渗透调节剂甜菜碱是否可缓解双氯芬酸钠带来的不良反应。将18只雄性Wistar大鼠分为三组：空白对照组不予任何处理，实验组每日经口给予20 mg/kg双氯芬酸钠，联合给药组每日给予20 mg/kg双氯芬酸钠与30 mg/kg甜菜碱的混合制剂。除检测血液学与生化指标外，本研究还对肝脏、肺脏、大脑、心脏及肾脏的组织病理学变化进行了观察。与空白对照组相比，双氯芬酸钠处理组动物的肝、肾、肺组织出现的组织病理学改变，在双氯芬酸钠联合甜菜碱处理组中则程度轻微甚至未出现。实验结果证实，甜菜碱可减轻双氯芬酸钠治疗相关的不良反应。