Genomic characterization of cisplatin response uncovers priming of cispla-tin-induced genes in a resistant cell line

We conducted a comprehensive genomic characterization of the cisplatin sensitive A2780 ovarian cancer cell line compared to A2780cis, its resistant derivative. The data includes eXcision Repair-sequencing (XR-seq) to map cisplatin repair,RNA-Seq to characterize gene expression and ATAC-Seq to detect changes in chromatin accessibility. Overall design: We performed XR-seq, RNA-Seq, ATAC-Seq in A2780 and A2780cis cells treated with 200µM ciplatin for 3h. XR-Seq: 3 A2780 replicates, 2 A2780cis replicates RNA-Seq: 3 replicates for each condition: A2780 control, A2780 + cisplatin, A2780cis control, A2780cis + cisplatin ATAC- Seq: 3 replicates for control samples, 2 replicates for cisplatin samples

本研究针对顺铂敏感型A2780卵巢癌细胞系及其耐药衍生株A2780cis开展了全面的基因组特征分析。
本次数据集包含用于绘制顺铂修复图谱的切除修复测序（eXcision Repair-sequencing, XR-seq）、用于表征基因表达特征的RNA测序（RNA-Seq），以及用于检测染色质可及性变化的转座酶可及性测序（ATAC-Seq）。
实验设计概况：我们对经200μM顺铂处理3小时的A2780及A2780cis细胞开展了XR-seq、RNA-Seq及ATAC-Seq实验。切除修复测序（XR-seq）：A2780细胞设置3次生物学重复，A2780cis细胞设置2次生物学重复；RNA测序（RNA-Seq）：所有实验分组均设置3次生物学重复，分组包括A2780对照组、A2780顺铂处理组、A2780cis对照组及A2780cis顺铂处理组；转座酶可及性测序（ATAC-Seq）：对照组样本设置3次生物学重复，顺铂处理组样本设置2次生物学重复。