Early Loss of Histone H2B Monoubiquitylation Alters Chromatin Accessibility and Activates Key Immune Pathways That Facilitate Progression of Ovarian Cancer. Early Loss of Histone H2B Monoubiquitylation Alters Chromatin Accessibility and Activates Key Immune Pathways That Facilitate Progression of Ovarian Cancer

We identified that downregulation of RNF20/H2Bub1 is involved in HGSOC progression through altering key immune signaling pathways. The goal of ATAC-seq analysis was to profile chromatin conformation in FTSEC cells (FT190 and FT194 cell lines) with RNF20 knockdown (shRNF20) or control shRNA. The data generated by ATAC-Seq was integrated with RNA-seq data analysis for the same samples. Overall design: Chromatin conformation profiles of FT190 and FT194 shRNF20 (RNF20 knockdown) or control shRNA cells were generated by using Illumina HiSeq 2500, in triplicates.

本研究鉴定发现，环指蛋白20/组蛋白H2B单泛素化（RNF20/H2Bub1）的表达下调可通过调控关键免疫信号通路，参与高级别浆液性卵巢癌（High-Grade Serous Ovarian Carcinoma, HGSOC）的疾病进展。
本研究中转座酶可及性测序（Assay for Transposase-Accessible Chromatin using sequencing, ATAC-seq）的分析目标为：对转染短发夹RNA（short hairpin RNA, shRNA）介导RNF20敲低（shRNF20）或对照shRNA的输卵管上皮细胞（Fallopian Tube Epithelial Cells, FTSEC）系FT190与FT194进行染色质构象谱分析。
本研究将ATAC-seq生成的测序数据与相同样本的RNA测序（RNA-seq）数据分析结果进行了整合分析。
整体实验设计：采用Illumina HiSeq 2500测序平台，对FT190与FT194细胞系中shRNF20（RNF20敲低）或对照shRNA处理的细胞进行染色质构象谱分析，每组设置3次生物学重复。