Gene regulation by Pax6 in the lens placode - E9.5 (2)

Wild type or Pax6 fx/fx; Le-Cre-positive (Pax6-/-) surface ectoderm from E9.5 mouse embryos was laser micodissected from three embryos of each genotype. Total RNA was purified, pooled for each genotype and triplicate samples were reverse transcribed and amplified using a NuGEN kit. cDNA was biotinylated and hybridized to Illumina Mouse6v2.0 bead arrays. Three wild type and three knockout embryos were used. The RNA from embryos of the same genotype was purified and pooled and triplicate samples were amplified and used for microarray analysis.

本数据集的样本源自E9.5天小鼠胚胎的表面外胚层，分为野生型（Wild type）与Pax6 fx/fx; Le-Cre阳性（Pax6基因敲除，Pax6-/-）两组，每组各取3枚胚胎进行激光显微切割。提取各组总RNA后按基因型混合，制备三份重复样本，采用NuGEN试剂盒完成逆转录与扩增。扩增得到的cDNA经生物素标记后，与Illumina Mouse6v2.0微珠芯片进行杂交。本实验共使用3枚野生型胚胎与3枚敲除型胚胎，相同基因型的胚胎总RNA经纯化、混合后，制备三份重复样本进行扩增，用于芯片微阵列分析。