RNA-sequencing of the oxygen induced retinopathy (OIR) model in C57BL/6 mice

Pathological retinal angiogenesis is one of the major causes of vision loss worldwide. The breakdown of blood vessels and aberrant vessels growth usually lead to hemorrhage, macular edema, fibrotic scar, and retinal detachment. OIR model serves as a proxy for human pathological retinal neovascularization such as proliferative diabetic retinopathy, retinal vein occlusion and retinopathy of prematurity. C57BL/6 mice were exposed to 75% O2 in a hyperoxic chamber from postnatal day P7 to P12 and then returned to room air. The greatest neovascular response occurred at postnatal 17 days (P17) when the mice are put back to room air condition for 5 days. In order to reveal potential genes that involve in pathological neovascularization, we performed transcriptomic analyses of retina of P17. 12 samples were analyzed: normoxic mice postnatal 17 (6 replicates), OIR mice postnatal 17 (6 replicates)

病理性视网膜血管生成（Pathological retinal angiogenesis）是全球范围内导致视力丧失的主要诱因之一。血管破裂与异常血管增生通常会引发出血、黄斑水肿、纤维化瘢痕及视网膜脱离。氧诱导视网膜病变（OIR）模型可作为人类病理性视网膜新生血管性疾病的替代模型，涵盖增殖性糖尿病视网膜病变、视网膜静脉阻塞及早产儿视网膜病变。将C57BL/6小鼠于出生后第7天（P7）至第12天（P12）期间置于75%氧浓度的高氧舱内，随后放回正常空气环境中饲养。当小鼠放回正常空气环境饲养5天后，于出生后第17天（P17）时可观测到最显著的新生血管应答反应。为了揭示参与病理性新生血管生成的潜在基因，我们对出生后第17天（P17）的小鼠视网膜组织开展了转录组分析。本研究共分析12个样本：正常氧环境饲养的出生后第17天小鼠视网膜样本（6个生物学重复），以及OIR模型小鼠出生后第17天视网膜样本（6个生物学重复）