A Myb transcription factor, PgMyb308-like, enhances the level of shikimate, aromatic amino acids, and lignins, but represses the synthesis of flavonoids and hydrolyzable tannins, in pomegranate (Punica granatum L.)

We cloned and characterized a subgroup S4 R2R3 Myb transcription factor, PgMyb308-like. Overexpressing PgMyb308-like in pomegranate hairy roots. Transcriptome analysis of PgMyb308-like-overexpressing hairy roots revealed reprogramming of cell wall-related genes, while overexpression of PgMyb308-like in Arabidopsis thaliana plants uncovered its distinct role in a different genetic and metabolic background. The results suggest that PgMyb308-like activates genes in the shikimate pathway and lignin biosynthesis, but suppresses those involved in the production of HTs and flavonoids. Overall design: Examination of 3-month old transgnic pomegranate hairy roots

本研究克隆并功能鉴定了S4亚群的R2R3类MYB转录因子（R2R3 Myb transcription factor）PgMyb308-like。我们将PgMyb308-like在石榴发根中进行过表达，对该过表达株系开展转录组分析后发现，其细胞壁相关基因的表达谱发生重编程；而在拟南芥（Arabidopsis thaliana）中过表达PgMyb308-like，则明确了其在异源遗传与代谢背景下的独特调控功能。研究结果表明，PgMyb308-like可激活莽草酸途径与木质素生物合成相关基因，但会抑制HTs与类黄酮合成相关基因的表达。实验设计概况：对生长3个月的转基因石榴发根进行检测分析。