whole brains from nicotinic acetylcholine receptor subunit mutant mice and littermate controls. Mus musculus

Studies using mice with beta4 nicotinic acetylcholine receptor (nAChR) subunit deficiency (beta4-/- mice) helped reveal the roles of this subunit in bradycardiac response to vagal stimulation, nicotine-induced seizure activity and anxiety. In order to identify genes that might be related to beta4-containing nAChRs activity, we compared the mRNA expression profiles of brains from beta4-/- and wild-type mice using Affymetrix U74Avr2 microarray. Keywords: knockout mice, nicotinic acetylcholine receptor Overall design: The experimental groups included six wild-type mice and five beta4-/- mice. All mice were 6-9 weeks old. Total RNA was extracted from whole brain tissue of the mice.

以β4烟碱型乙酰胆碱受体（nicotinic acetylcholine receptor, nAChR）亚基缺陷小鼠（β4-/-小鼠）为模型的研究，有助于阐明该亚基在迷走神经刺激所致心动徐缓反应、尼古丁诱导的癫痫发作活动及焦虑中的作用。为鉴定可能与含β4亚基的nAChRs活性相关的基因，本研究采用Affymetrix U74Avr2基因芯片，对比了β4-/-小鼠与野生型小鼠的全脑组织mRNA表达谱。关键词：基因敲除小鼠，烟碱型乙酰胆碱受体。实验设计：实验组包含6只野生型小鼠与5只β4-/-小鼠，所有小鼠均为6~9周龄。从小鼠全脑组织中提取总RNA。