Amplicon-sequencing over the Anopheles gambiae CP, AP2 and Aper1 loci after non-autonomous gene drive

We co-opted the native regulatory sequences of three midgut-specific loci of the malaria vector Anopheles gambiae to host a prototypical antimalarial molecule and guide-RNAs encoded within artificial introns, that support efficient gene drive. We assessed the homing rate and resistance allele formation in male and female transhemizygotes, as well as in the G3 wild-type and the vasa-Cas9 controls.

我们利用疟疾媒介冈比亚按蚊（Anopheles gambiae）的三个中肠特异性基因座的天然调控序列，将原型抗疟分子与向导RNA（guide-RNAs）编码于人工内含子中，以此构建可实现高效基因驱动的转基因系统，并对雌雄半合子转基因个体、G3野生型及vasa-Cas9对照组的归巢率与抗性等位基因形成情况开展了评估。