Transcriptional profiling of germinal center B cells and isotype switched memory B cells (scRNA-Seq). Transcriptional profiling of germinal center B cells and isotype switched memory B cells (scRNA-Seq)

Germinal center B cells were analyzed for memroy B cell gene signature to identify a memory B cell precursor population. Sorted isotype-switched memory B cells were used to confirm the identified memroy B cell precursor population Overall design: Antigen specific germinal center B cells (CD19+GL7+IgD-EphrinB1+WNV WT DIII tetramer+) and polyclonal memory B cells (CD19+IgD-IgM-CD80+CCR6+) were sorted from 30 pooled 8-12 week old C57BL/6 mice that were vaccinated with Innovator WNV vaccine 7 days prior.

本研究针对生发中心B细胞（germinal center B cell）的记忆B细胞基因特征开展分析，以鉴定记忆B细胞前体细胞群。分选得到的同型转换记忆B细胞被用于验证所鉴定出的记忆B细胞前体细胞群。
总体实验设计：从采样前7天接种Innovator WNV疫苗的30只混合的8~12周龄C57BL/6小鼠中，分选出抗原特异性生发中心B细胞（CD19+GL7+IgD-EphrinB1+WNV野生型DIII四聚体+）以及多克隆记忆B细胞（CD19+IgD-IgM-CD80+CCR6+）。