Specific binding of Cry1Ac and Cry1AcMod toxins to BBMV from resistant (AZP-R) and susceptible (APHIS-S) larvae of P. gossypiella.

The left lane of each pair shows total binding with no competitor (-). The right lane shows non-specific binding performed in the presence of 1000-fold excess of unlabeled competitor (Cry1Ac or Cry1AcMod, respectively) (+). The numbers under the bands with competitor (+) represent the optical density of the bands relative to the corresponding bands on the left without competitor (-) (set to 100). Total binding minus non-specific binding equals specific binding.

每组泳道对的左侧泳道展示未添加竞争剂（标记为(-)）时的总结合量；右侧泳道则展示添加1000倍过量未标记竞争剂（分别对应Cry1Ac或Cry1AcMod，标记为(+)）时的非特异性结合量。带(+)标记的竞争剂组条带下方的数值，代表该条带光密度相对于左侧未加竞争剂(-)组对应条带的比值（后者设为100）。总结合量减去非特异性结合量，即为特异性结合量。