Proteomics of MRSA biofilms under Elasnin Treatment

Due to the great challenge in treating biofilms, there is an urgent need for novel and effective antibiofilm compounds. Through bioassay-guided isolation of bioactive compounds from Actinobacteria, we identified elasnin as a potent biofilm-targeting compound against methicillin-resistant Staphylococcus aureus (MRSA). Elasnin effectively inhibited biofilm formation and eradicated pre-formed biofilms of MRSA. To gain more insights on how elasnin eradicate MRSA biofilms, we conducted proteomics study on MRSA biofilm cells under elasnin treatment compared to the untreated cells.

鉴于生物膜（biofilm）的临床治疗面临极大挑战，研发新型高效抗生物膜化合物的需求尤为迫切。本研究通过生物活性导向分离法从放线菌（Actinobacteria）中分离活性化合物，鉴定出elasnin为一种可靶向对抗耐甲氧西林金黄色葡萄球菌（MRSA）生物膜的强效活性物质。Elasnin能够有效抑制MRSA生物膜的形成，并可清除已形成的MRSA生物膜。为深入阐明elasnin清除MRSA生物膜的作用机制，我们以未处理的MRSA生物膜细胞为对照，对经elasnin处理的MRSA生物膜细胞开展了蛋白质组学（proteomics）研究。