EdU Optimization - Optimized Procedure Example

Flow cytometry data on mouse fecal bacteria incubated for three hours with (or without) 20 uM 5-ethnyl-2'-deoxyuridine (EdU), a thymidine analogue, in a 1:1 ratio of reduced Brain Heart Infusion (rBHI) broth and fecal slurry (FS), to capture the actively replicating bacteria of healthy mice. <br> The bacterial cells underwent a click reaction with Alexa Fluor 647 azide (APC channel) to make newly replicated bacteria fluoresece. They were also stained with Sybr Green I nucleic acid stain (FITC channel) to capture all bacteria. <br> In the file names: w = with wo = without AF = Alexa Fluorphore 647本数据集包含健康小鼠粪便细菌的流式细胞术（Flow Cytometry）数据：将粪便细菌置于还原型脑心浸液（rBHI）肉汤与粪便悬液（FS）按1:1体积比混合的体系中，与20 μM 5-乙炔基-2'-脱氧尿苷（5-ethynyl-2'-deoxyuridine, EdU，一种胸苷类似物）共孵育3小时，设置添加与不添加EdU的两组对照，以捕获健康小鼠体内的活跃复制细菌。 细菌细胞将与亚历克斯弗卢尔647叠氮化物（APC通道）进行点击化学反应，使新复制的细菌发出荧光；同时采用Sybr Green I核酸染料（FITC通道）进行染色，以标记全部细菌。 文件名说明：w代表添加EdU组，wo代表不添加EdU组，AF代表亚历克斯弗卢尔647荧光基团。