Aging-exacerbated demyelinating injury is associated with microglia-derived reactive oxygen species: alleviation by indapamide. Aging-exacerbated demyelinating injury is associated with microglia-derived reactive oxygen species: alleviation by indapamide

We sequenced the transcriptome of young (6-8 week) and middle-aged (8-10 month old) C57BL/6 female mice that were naïve or had injured ventrolateral spinal cord white matter. We found several differentially expressed genes, many suggesting an altered immune response to injury with advancing age. Overall design: Naïve or injured (72 hours after the local injection of lysolecithin) ventrolateral white matter of the spinal cord was isolated using laser capture microdissection. RNA was then extracted using RNeasy MiniKit (Qiagen) as per manufacturer’s instruction and sequenced using the Illumina NextSeq500. Tissue was pooled from 5 animals per condition and 3 replicates were sequenced for the analysis.

本研究对年轻（6~8周龄）及中年（8~10月龄）的C57BL/6雌性小鼠开展转录组测序，这些小鼠分为未造模组与脊髓腹外侧白质损伤组。本研究筛选得到多个差异表达基因，其中多数提示随着年龄增长，机体对脊髓损伤的免疫应答发生了改变。整体实验设计：通过激光捕获显微切割技术，分离未造模或造模（局部注射溶血卵磷脂72小时后）的脊髓腹外侧白质组织；随后按照制造商说明书，使用RNeasy MiniKit（Qiagen公司）提取RNA，并通过Illumina NextSeq500平台完成测序。每组均取自5只小鼠的混合组织，且设置3次生物学重复用于后续分析。