Knock down of C11orf67 by shRNA in SUM52PE

SUM52PE cells were lentivirally transduced with PKLO empty vector control (Sigma SHC001 MISSION PLKO.1-PURO CONTROL VECTOR) or shRNA specific for C11orf67 (Sigma Clone ID: NM_024684.2-280s21c1 SHCLND-NM_024684 TRCN0000263814) in biological triplicates. Differentially regulated targets by the shRNA relative to empty vector control were determined. Overall design: triplicates

将SUM52PE细胞分为两组，各设置三次生物学重复，分别采用PKLO空载体对照（Sigma公司SHC001 MISSION PLKO.1-PURO对照载体）或靶向C11orf67的短发夹RNA（short hairpin RNA，shRNA，Sigma公司克隆编号：NM_024684.2-280s21c1、SHCLND-NM_024684、TRCN0000263814）进行慢病毒转导。随后鉴定相较于空载体对照，该shRNA所调控的差异表达靶标。实验整体设计：设置生物学重复三次。