CD5 alters gene transcription in Jok-1 B cells

To assess the impact of CD5 on the transcriptome in B cells, we studied changes in mRNA levels in Jok-E1A/Jok-E1B cells compared with Jok-1 cells. Gene expression was performed in untransfected CD5-negative hairy B cell leukemia line (Jok-1), transfected with cDNA for either the membrane isoform of CD5 (E1A; Jok-E1A) or the cytoplasmic E1B isoform (Jok-E1B). Three biological replicates were performed.

为评估CD5对B细胞转录组（transcriptome）的影响，本研究对比分析了Jok-E1A、Jok-E1B与Jok-1细胞的mRNA水平变化。实验所用细胞包括未转染的CD5阴性多毛B细胞白血病细胞系Jok-1，以及分别转染CD5膜结合型亚型（E1A，对应Jok-E1A）或胞质型E1B亚型（对应Jok-E1B）互补DNA（cDNA）的细胞，以此开展基因表达分析。本实验共设置三次生物学重复。