Differential transcriptome responses in human THP-1 macrophages following exposure to T98G and LN-18 human glioblastoma secretions: a simplified bioinformatics approach to understanding patient-glioma-specific effects on tumor-associated macrophages. Differential transcriptome responses in human THP-1 macrophages following exposure to T98G and LN-18 human glioblastoma secretions: a simplified bioinformatics approach to understanding patient-glioma-specific effects on tumor-associated macrophages

We first describe a methods approach to differentiating THP-1 monocytes to macrophages with M0-like genotype. Then we describe the effect that different glioblastoma supernatants have on the gene expression profiles of macrophages, suggesting gliomas should be considered different diseases. Overall design: Differential gene expression analysis of RNA-seq data for THP-1 monocytes, differentiated macrophages, and those same macrophages treated with different glioma supernatants.

我们首先介绍一种将THP-1单核细胞诱导分化为具有M0样基因型巨噬细胞的方法学方案。随后我们阐述了不同胶质母细胞瘤细胞上清液对巨噬细胞基因表达谱的影响，该结果提示胶质瘤应被视作不同的疾病类型。整体实验设计：对THP-1单核细胞、诱导分化得到的巨噬细胞，以及经不同胶质瘤上清液处理的上述巨噬细胞的RNA-seq数据进行差异基因表达分析。